Do anybody know the meaning of "holy cow"?
I have read this expression in the book "Fifty shades of Grey" it seemed like a surprise expression

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It's a very common expression. You can hear it in almost every english speaking movie and tv series episode.

And indeed, it's an exclamation of surprise.

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Thank you for answer me this doubt was driving me crazy.

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You've got it correct. It is an exclamation, usually surprise. Like "oh, wow!"