Pesquise entre vários professores de Inglês...
Postagens
my C.V. continue
3. Project “fundamental research on pathogenesis and intervention of myopia”
I set up a customer-made electroretinogram system. With LabVIEW programming, I built a Graphical User Interface, in this way we can control a LED driver circuit, a DC preamplifier, and a Ganzfeld work together and synchronously. With this ERG system, my colleagues examined the myopia model animals still have normal vision response rather than blindness. Then this system still works today. In addition, I created a mathematical formula, by which we could quantitatively evaluate the different contributions between axial length and corneal radius of curvature to the refractive state changes of mice eyes. See also publication list [3].
TEACHING EXPERIENCE
Except for time spent in studying for academic degrees, I worked as a faculty member at School of Life Science and Technology, Changchun University of Science and Technology. I took charge of an experiment course that helped undergraduate students in biomedical engineering program grasp medical instruments design principles. During that time, I improved my presentation skills and accumulated practical experience in electronics and optics technology.
EXPERTISE
Patch clamp recording (loose patch, whole cell, intracellular Iontophoresis)
Immunohistochemistry
Fluorescence/confocal/two-photon microscopy
Western blot
Optogenetics
Electroretinogram
LabVIEW programing
8051 serial microcontrollers
Optical design (Prior experience but rusty)
Multi-electrode array recording (just worked with, not skillful)
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my C.V.
Guo-Zhong
EDUCATION
Fudan University, Institutes of Brain Science, State Key Laboratory of Medical neurobiology Shanghai, CHINA
Ph.D. in neurobiology Sept. 2012 ~ Oct. 2017
Advisor: Prof. XXX, Prof. XXX
Title of dissertation: “An optogenetic study of non-glutamatergic excitatory inputs to mouse retinal ganglion cells”.
Changchun University of Science and Technology, School of Photo-electronic Engineering Changchun, CHINA
Master in optical engineering, Sept. 2008 ~ Apr. 2011
Changchun University of Science and Technology, School of Life Science and Technology Changchun, CHINA
Bachelor’s in biomedical engineering, Sept. 1999 ~ Jul. 2003
RESEARCH EXPERIENCE
1. project “Optogenetic dissection of inhibitory neural circuits in the inner retina”
I examined 7 transgene mouse lines’ specificity by immunohistochemistry. Finally, I picked up VGAT-ChR2-EYFP mouse as the model animal for analyzing the inner retina inhibitory neural circuits and further identified the transgene Channelrhodopsin-2’s functionality by patch clamp and optogenetics. By chance, I found it is common to see ganglion cells having not only IPSC, but also EPSC, in the condition of blocking glutamatergic transmission, even blocking ACh transmission, when a population of amacrine cells was optogenetically activated. These results propose a possibility of the existence of an unknown co-transmission system in the retina amacrine cells. We have published a part of this work in Neuroscience in November 2017 (see also publication list [2]), and the remaining work is ongoing by my labmate.
2. Project “the mechanism of orexin modulating excitatory and inhibitory transmission in the retina ganglion cells”
I investigated the expression of orexins and their receptors in rat retina, localized the orexin A/B and its receptor1/2 in the inner retina circuits with immunofluorescence labelling techniques. This work opened a serial research projects about orexin in the retina in our lab. See also publishing list [4,1].
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