There are several ways to say this. "I am separated from my wife" or "my wife and I are separated", "I got separated from my wife" and "I separated from my wife" are also possible.
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Susan is right, there are several correct ways to say it. However "separate my wife" is incorrect; that sounds like you're going to cut her into pieces, haha!
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I am separate with my wife .. or i separted from my wife . i have separated from my wife