No, that would sound awkward, though your meaning would be clear, it is not a native way of saying it. We would say I have no sisters, I don't have any sisters, I haven't got any sisters, I've got no sisters.

In American English, we don't, while in British English, you could, although you would normally include 'any' — 'I haven't any sisters'

You probably don't need any more answers to this, but here's mine anyway: No. It's not natural at all. The negative form of 'have' as a main verb is NOT simply 'haven't'. If you use 'have' to refer to an action ( a dynamic verb) , you have one option: 'I don't have a big breakfast on weekdays.' If you use 'have' as a stative verb, usually indicating possession, you have two : 'I don't have...' 'I haven't got ...' It's also usual to include 'any/no', which in fact gives you a third option: 'I don't have any sisters' 'I haven't got any sisters' 'I have no sisters'

I do not have any sisters. I don't have any sisters. Not- I haven't any sisters because that would mean: I have not sisters. Hope this helps

No, but you can say 'I haven't any sisters'. You must use any if you want to use this structure which is alittle more formal and a little old fashioned but perfectly acceptable.