Don't bet on it=it is impossible?:)
Don't bet on it=it is impossible?:) Are they same?

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Thanks a lot:)

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Let me use them both in a conversation:

Two friends watch a very beautiful girl leave the building they are in.
"Do you think we'll ever see her again?"
"Don't bet on it."

Meaning it is very unlikely.

2.
"Do you think I could fly like Superman?"
"No, it's impossible."

Meaning it will never happen.

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Don't bet on it means implies that the speaker thinks it is very unlikely.  Although it may be possible, it isn't worth risking anything over.