分かりました。私は日本語を勉強

"On your mark" is like saying "Get into your starting position."

「 位置に着いて！ 用意！ ドン！」が恐らく英語の、 「On your mark! Get set! Go!」にあたるかと思います。

you mean the anime?i have seen it before~

on your mark... get set... go! it is something called out to competitors at the beginning of a running race