Does this make sense? "Я тебя не разыгрываю", meaning like.. I'm not joking with you, I'm serious.

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that's ok! You can also say: Я не шучу

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I'm not sure if 'to joke with...' has this meaning:

"kid., v intr.:
....
2. To deceive in fun; fool: I could only hope they were kidding me when they said my car had been stolen."

But it is this, specificaly.

btw, does 'joke with' mean the same?

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You can say: I mean it! I'm serious! - Я не шучу! Я серьёзно!

Это не шутка! - It's not a joke!
Я не шучу с тобой, я тебя не разыгрываю! Это не розыгрыш!

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right, also could be translated as "I'm not playing with u"

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"Я тебя не разыгрываю"
or
"Я не шучу"
or
"Я серьёзно"
or
"Никаких шуток"