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Abstract: The effects of 5% mass of biochar and 5% pig manure alone or in combination on exogenous Cu pollution (0,200,400 mg • kg-1) soils on the growth and Cu formation were studied by in-pot experiment. The results showed that the application of pig manure could significantly increase the biomass of Yongcao in red soil with low nutrient content (the average was about 59.4% higher than that of CK), and promoted the absorption and accumulation of Cu in Yongqing. (60%) were increased by 62.0%, 70.0% (200 mg • kg-1) and 74.5%, respectively, compared with the CK (CK) 78.3% (400 mg • kg-1). In the control (CK), the exchangeable state, carbonate-bound state, iron-manganese oxide bound state and organic matter binding content were all small, and copper treatment increased the above parameters. The content of Cu in various Cu-polluted soils varied with the treatment, and intermolecular and organic matter-bound Cu in the soil of single-cropping manure was used. The organic matter-bound Cu found in the soil of a single application of biochar mixed with acid-bound Cu and organic-bound Cu were significantly increased.