In "자전거 탈 줄 알아요?"
탈 줄 what does the two mean? and their uses?
and would it mean the same if I added 자전거는?

Question

Answer

To know (how to): ~ㄹ/을 줄 알다
By placing ~ㄹ/을 줄 알다 after a verb, you can indicate that one “knows how” to do that action. For example:
각도를 잴 줄 알아요 = I know how to measure the angle
저는 그것을 할 줄 알아요 = I know how to do that

자전거를 타다 means "ride a bicycle"
자전거(를) 탈 줄 알아요? means "Do you know how to ride a bicycle?"
자전거는 탈 줄 알아요? means "(I know that you cannot ride something). Do you know how to ride a bicycle?"
자전거도 탈 줄 알아요? means "(I know that you can ride something). Do you also know how to ride a bicycle?"