Not sarcastic.
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Use it playfully.
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It is "taking a liberty", doing something that is not proper. Calling someone a name. Saying something inappropriate.
Most often with teh purpose of making fun of someone.
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Don't give me any of your cheek. = don't be cheeky. = almost= mind your manners.
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Tone alone tells you whether the person is serious.
Child steals a muffin.
Ohh, you cheeky blighter. Come here. I'll teach you a lesson.
Most often with children.